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Image Search Results
Journal: Scientific Reports
Article Title: The third-generation anti-CD30 CAR T-cells specifically homing to the tumor and mediating powerful antitumor activity
doi: 10.1038/s41598-022-14523-0
Figure Lengend Snippet: Proliferation and cytotoxicity of anti-CD30 CAR T-cells. (A) Schematic illustration of the anti-CD30 CAR (CD30-28z and CD30-28BBz) constructs. (B) Percentage of CAR+ T cells during the CAR T-cells (CD30-28z and CD30-28BBz) in vitro culture. (C) Total cell number at the time of transduction of anti-CD30 CAR T-cells (CD30-28z and CD30-28BBz). (D) The transgene copy number per one million CARS+ cells at the time of transduction of anti-CD30 CAR T-cells (CD30-28z and CD30-28BBz). (E) FACS was used to detect CD30 expression in negative cells (Raji, Jurkat and K562) and positive cells (L428 and L540). Gray line: isotype control; Blue line: K562; Red line CD30 antibody and K562 overexpression CD30. (F) The calcein release assay was used for in vitro cytotoxicity testing at 3 different effectors: target ratios on CD30 negative cell lines as indicated. (G) The calcein release assay was used for in vitro cytotoxicity testing at 3 different effectors: target ratios on CD30 positive cell lines and K562-CD30 as indicated. (H–I) Anti-CD30 CAR T-cells were co-cultured with L428 cells. After 24 h, supernatants were collected for cytokine(IFNγ and IL-6) production analysis. All experiments were performed at least three independent times, and *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001. In the ( B,C,D,F,G ) black bar: T cells; green bar: CD30-28z; red bar: CD30-28BBz.
Article Snippet: L428 and L540(Human Hodgkin’s lymphoma cell line) were purchased from the
Techniques: Construct, In Vitro, Transduction, Expressing, Control, Over Expression, Release Assay, Cell Culture